Adenoviruses are non-enveloped icosohedral viruses
containing double-stranded DNA.
There are 49 immunologically distinct types (6 subgenera : A through F) that can cause human infections.
Adenoviruses most commonly cause respiratory illness
; however, depending on the infecting serotype, they
may also cause various other illnesses such as gastroenteritis, conjunctivitis, cystitis, and rash illness.
 Symptoms of respiratory illness caused by adenovirus
infection range from the common cold syndrome to
pneumonia, croup, and bronchitis. Patients with
compromised immune systems are especially susceptible
to severe complications of adenovirus infection.
Acute respiratory disease (ARD), first recognized among
military recruits during World WarĄ±, can be caused by
adenovirus infections during conditions of crowding and
stress.
 Antigen detection, polymerase chain reaction assay,
virus isolation, and serology can be used to identify
adenovirus infections. Viro-Capture^TM  Adenovirus
Detection Kit is a qualitative immunoassay kit for the detection and identification of adenoviruses in fecal specimen or adenovirus infected cell culture supernatant.

The Viro-Capture^TM Adenovirus Detection Kit is antigen capturing enzyme immunoassay intended for the detection of Adenovirus antigens in human feces or supernatant obtained form Adenovirus infected cell monolayer.





All reagents provided are stored at 4°C. Refer to the expiration date on the label.
Each kit contains reagents sufficient materials for 96 determinations.

1. MICROPLATE (Cat. No VC-PA01) ---------------------------------------------------------- 96 well

    One 96 well microplate in a frame (12 x 8 well breakapart microwell strips). Pre-coated with     mouse adenovirus monoclonal antibody against group A Adenovirus in a reusable foil bag.

2. SAMPLE DILUENT (Cat. No VC-P001) ----------------------------------------------------- 100 ml
   
    Tris buffered saline containing thimerosal agent and red dye.

3. WASHING BUFFER(25X conc) (Cat. No VC-P002) ----------------------------------------- 40 ml
    
    Tris buffered saline containing thimerosal agent and detergent

4. POSITIVE CONTROL (Cat. No VC-PA04) ---------------------------------------------------- 2 ml

    Inactivated Adenovirus. Ready to use

5. CONJUGATE (Cat. No VC-PA05) ------------------------------------------------------------ 11 ml

    Polyclonal antibody against Adenovirus conjugated with horseradish peroxidase in a buffered     solution containing  antimicrobial agent and red dye. Ready to use

6. SUBSTRATE/CHROMOGEN (Cat. No VC-P003) -------------------------------------------- 11 ml

    Substrate buffer containing TMB. Ready to use

7. STOP SOLUTION (Cat. No VC-P004) ------------------------------------------------------- 11 ml

    0.5N sulfuric acid. Ready to use





Prepare a 1 : 10 dilution of stool by adding 0.1 gram to 1ml of sample dilution buffer. Mix well
and centrifuge at 3000rpm, 4°C for 20min. Supernatant is used for the test. For diarrhea stools
a lower dilution may be used (i.e., 1 : 5 dilution).





1. Add 2 drops (100ul) of positive control or samples and add 2 drops of enzyme conjugate.

2. Incubate at 25°C for 60min.

3. Wash wells 5 times by using 1x washing buffer.

4. Add 2drops of substrate solution.

5. Incubate at 25^oC for 15min.

6. Add 1 drop of stop solution and read absorbance at 450nm wavelength.





Positive   : Absorbance reading of 0.2 and above indicates the sample contains Adenovirus                 antigen.

Negative : Absorbance reading less than 0.2 indicates the sample does not contain detectable                 levels of Adenovirus antigen.